The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items had been digested and cloned right into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was launched by the quick https://chancevphev.sharebyblog.com/26467294/sr-4835-an-overview